Effectiveness of Permeable Reactive Bio-Barriers for Bioremediation of an Organohalide-Polluted Aquifer by Natural-Occurring Microbial Community

In this study, a bioremediation approach was evaluated for the decontamination of an aquifer affected by release organohalides industrial landfill. After preliminary physicochemical and microbiological characterization landfill groundwater, stimulation natural organohalide respiration addition reducing substrate (i.e., molasse) tested both at microcosm field scales, placement anaerobic permeable reactive bio-barrier. Illumina sequencing cDNA 16S rRNA gene revealed that organohalide-respiring bacteria genera Geobacter, Sulfurospirillum, Dehalococcoides, Clostridium Shewanella were present within microbial community, along with fermentative Firmicutes Parvarchaeota. Microcosm experiments confirmed presence active attenuation, which boosted substrate. Field tests showed bio-barrier decreased concentration chloroethenes rate 23.74 kg d?1. Monitoring biomarkers qPCR native populations involved in dechlorination process, although their specific role still needs to be clarified. The accumulation lower-chloroethenes suggested need future improvement supporting bacterial vinyl-chloride oxidation, achieve complete degradation chloroethenes.